College of Arts and Sciences
Master of Science in Biology (MS)
NACO controlled Corporate Body
University of North Florida. Department of Biology
Dr. Terri N. Ellis
Dr. Judith D. Ochrietor
Dr. Laura Lewis-Tuffin
Dr. Cliff Ross
Dr. Daniel Moon
Klebsiella pneumoniae is an opportunistic pathogen responsible for lobar pneumoniae, liver abscess, and septicemia. Clinical isolates are found to be extended spectrum beta lactamase positive with differential expression of the two classical porins, OmpK35 and OmpK36. Porin loss is associated with increased minimum inhibitory concentrations of beta lactam, cephalosporin, and carbapenem antibiotics that target the peptidoglycan. However, little is known about how porin loss affects other aspects of the cell envelope. The focus of this study was to characterize clinical isolates exhibiting differential porin expression and determine if the cumulative changes altered the resistance to phagocytosis by macrophages. The results support the hypothesis that porin loss significantly impacts the overall cell envelope composition, which in turn alters interactions with macrophages.
Brunson, Debra Nickole, "Loss of outer membrane porins in clonally related clinical isolates of Klebsiella pneumoniae modifies the bacteria; resulting in altered resistance to phagocytosis by macrophages" (2017). UNF Graduate Theses and Dissertations. 724.