All Volumes (2001-2008)


Volume VII, 2008

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It has been hypothesized that the cell adhesion molecule Basigin and the lactate transporter monocarboxylate transporter-1 (MCT1) interact to form a lactate shuttle necessary to fuel photoreceptor neurons in the retina. The purpose of this study was to characterize the interaction between the two proteins. Specifically, the amino acids within MCT1 that interact with Basigin were investigated. MCT1 is a large protein. Therefore, the complementary DNA (cDNA) for MCT1 was divided in half via the polymerase chain reaction (PCR) and each half was cloned into a bacterial expression vector to generate protein probes. The probes were then used to test for binding to Basigin isolated from mouse retina via enzyme-linked immunosorbant assay (ELISA). The results of the ELISA studies indicate that amino acids within the second half, or carboxy (C)-half, of MCT1 are used in the interaction with Basigin. Future studies will be aimed at determining which amino acids within the C-half of MCT1 are specifically involved.

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