Faculty Sponsor

Dr. Fatima Rehman, PhD

Faculty Sponsor College

College of Arts and Sciences

Faculty Sponsor Department

Biology

Location

SOARS Virtual Conference

Presentation Website

https://unfsoars.domains.unf.edu/saffron-crocus-sativus-extract-has-anticancer-activity-through-inhibition-of-migration-and-invasion-potential-of-breast-cancer-cells/

Keywords

SOARS (Conference) (2020 : University of North Florida) -- Posters; University of North Florida. Office of Undergraduate Research; University of North Florida. Graduate School; College students – Research -- Florida – Jacksonville -- Posters; University of North Florida – Undergraduates -- Research -- Posters; University of North Florida. Department of Biology -- Research -- Posters; Biology, Physics, and Chemistry -- Research – Posters

Abstract

Introduction: Saffron is a spice which has been used throughout history for various therapeutic properties. Recently, it has also been documented to have significant anticancer effect on primary tumors. However, limited studies are found studying its effect on metastatic potential of cancers. The goal of our study was to explore the effect of saffron on migration and metastatic potential of breast cancer cells.

Methods: MDA468 breast carcinoma cells were treated with varying concentrations of Saffron and examined using Q-phase holographic time-lapse microscopy to measure the movement of cells over time. The anticancer activity of Saffron was confirmed using cell viability assays. Invasion and metastatic potential was studied using boyden-chamber and scratch wound assays. Finally, tumorigenicity of the cell line was examined through colony formation assays in the presence and absence of Saffron.

Results: Q-phase microscopy revealed reduction in projections and significantly reduced movements in cells after Saffron treatment. The inhibition of migration, cell mobility and invasion by Saffron was confirmed in scratch wound and boyden chamber assays (p

Conclusions: Our results found saffron to be effective in decreasing breast cancer carcinoma cells MDA468’s viability, migration, and tumorigenicity. Future studies will examine its mechanism of action and will focus on delineating the pathways involved in its anticancer effects.

Included in

Biology Commons

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Apr 8th, 12:00 AM Apr 8th, 12:00 AM

Saffron (Crocus sativus) extract has anticancer activity through inhibition of migration and invasion potential of breast cancer cells

SOARS Virtual Conference

Introduction: Saffron is a spice which has been used throughout history for various therapeutic properties. Recently, it has also been documented to have significant anticancer effect on primary tumors. However, limited studies are found studying its effect on metastatic potential of cancers. The goal of our study was to explore the effect of saffron on migration and metastatic potential of breast cancer cells.

Methods: MDA468 breast carcinoma cells were treated with varying concentrations of Saffron and examined using Q-phase holographic time-lapse microscopy to measure the movement of cells over time. The anticancer activity of Saffron was confirmed using cell viability assays. Invasion and metastatic potential was studied using boyden-chamber and scratch wound assays. Finally, tumorigenicity of the cell line was examined through colony formation assays in the presence and absence of Saffron.

Results: Q-phase microscopy revealed reduction in projections and significantly reduced movements in cells after Saffron treatment. The inhibition of migration, cell mobility and invasion by Saffron was confirmed in scratch wound and boyden chamber assays (p

Conclusions: Our results found saffron to be effective in decreasing breast cancer carcinoma cells MDA468’s viability, migration, and tumorigenicity. Future studies will examine its mechanism of action and will focus on delineating the pathways involved in its anticancer effects.

https://digitalcommons.unf.edu/soars/2020/spring_2020/74

 

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