Faculty Sponsor
Dr. Matthew Gilg
Faculty Sponsor College
College of Arts and Sciences
Faculty Sponsor Department
Biology
Location
SOARS Virtual Conference
Presentation Website
https://unfsoars.domains.unf.edu/2021/posters/no-evidence-of-heteroplasmy-found-in-mitochondrial-dna-of-fundulus-heteroclitus-and-fundulus-grandis-hybrids/
Keywords
SOARS (Conference) (2021 : University of North Florida) – Archives; SOARS (Conference) (2021 : University of North Florida) – Posters; University of North Florida -- Students -- Research – Posters; University of North Florida. Office of Undergraduate Research; University of North Florida. Graduate School; College students – Research -- Florida – Jacksonville – Posters; University of North Florida – Undergraduates -- Research – Posters; University of North Florida. Department of Biology -- Research – Posters; Project of Merit Award
Abstract
Project of Merit Winner
Hybridization between closely related species is fairly common in the wild, occurring in areas designated as hybrid zones. In these areas, the ranges of two species overlap enough to allow them to produce hybrid offspring, often in a directional manner, where females of one species are more successful at the production of hybrid offspring than the other. The direction of such a cross can be detected via mitochondrial DNA (mtDNA), which in many species is inherited entirely from the mother due to egg-sperm recognition mechanisms, which can purge male mtDNA from the offspring. However, in some instances of hybridization however, the mtDNA from the father remains in the offspring with the mtDNA from the mother, resulting in heteroplasmy. Two species of killifish, Fundulus heteroclitus and F. grandis, hybridize in a narrow portion of northeastern Florida and have shown strong directionality in laboratory crosses. This investigation sequenced the mitochondrial gene cytochrome-b and repeated previous restriction digest experiments to determine whether these killifish experienced paternal leakage, and if heteroplasmy occurred more frequently in a give direction. The study found that there was no evidence of heteroplasmy amongst the hybrids examined, and therefore showed no directionality in which offspring were heteroplasmic. In addition, results upheld a strict pattern of maternal inheritance, as offspring always matched the mtDNA genotype of their mother.
Rights Statement
http://rightsstatements.org/vocab/InC/1.0/
Included in
No Evidence of Heteroplasmy found in Mitochondrial DNA of Fundulus heteroclitus and Fundulus grandis hybrids
SOARS Virtual Conference
Project of Merit Winner
Hybridization between closely related species is fairly common in the wild, occurring in areas designated as hybrid zones. In these areas, the ranges of two species overlap enough to allow them to produce hybrid offspring, often in a directional manner, where females of one species are more successful at the production of hybrid offspring than the other. The direction of such a cross can be detected via mitochondrial DNA (mtDNA), which in many species is inherited entirely from the mother due to egg-sperm recognition mechanisms, which can purge male mtDNA from the offspring. However, in some instances of hybridization however, the mtDNA from the father remains in the offspring with the mtDNA from the mother, resulting in heteroplasmy. Two species of killifish, Fundulus heteroclitus and F. grandis, hybridize in a narrow portion of northeastern Florida and have shown strong directionality in laboratory crosses. This investigation sequenced the mitochondrial gene cytochrome-b and repeated previous restriction digest experiments to determine whether these killifish experienced paternal leakage, and if heteroplasmy occurred more frequently in a give direction. The study found that there was no evidence of heteroplasmy amongst the hybrids examined, and therefore showed no directionality in which offspring were heteroplasmic. In addition, results upheld a strict pattern of maternal inheritance, as offspring always matched the mtDNA genotype of their mother.
https://digitalcommons.unf.edu/soars/2021/spring_2021/66