CORP: Gene delivery into murine skeletal muscle using in vivo electroporation

Authors

David C. Hughes, Division of Endocrinology and Metabolism, Department of Internal Medicine, Carver College of Medicine, University of Iowa, Iowa City, Iowa.
Justin P. Hardee, Centre for Muscle Research (CMR), Department of Anatomy and Physiology, The University of Melbourne, Melbourne, Victoria, Australia.
David S. Waddell, University of North FloridaFollow
Craig A. Goodman, Centre for Muscle Research (CMR), Department of Anatomy and Physiology, The University of Melbourne, Melbourne, Victoria, Australia.

Document Type

Article

Publication Date

7-1-2022

Subject Area

Animals; DNA; Electroporation (methods); Gene Transfer Techniques; Genetic Therapy; Mice; Muscle, Skeletal; Plasmids (genetics)

Abstract

The strategy of gene delivery into skeletal muscles has provided exciting avenues in identifying new potential therapeutics toward muscular disorders and addressing basic research questions in muscle physiology through overexpression and knockdown studies. In vivo electroporation methodology offers a simple, rapidly effective technique for the delivery of plasmid DNA into postmitotic skeletal muscle fibers and the ability to easily explore the molecular mechanisms of skeletal muscle plasticity. The purpose of this review is to describe how to robustly electroporate plasmid DNA into different hindlimb muscles of rodent models. Furthermore, key parameters (e.g., voltage, hyaluronidase, and plasmid concentration) that contribute to the successful introduction of plasmid DNA into skeletal muscle fibers will be discussed. In addition, details on processing tissue for immunohistochemistry and fiber cross-sectional area (CSA) analysis will be outlined. The overall goal of this review is to provide the basic and necessary information needed for successful implementation of in vivo electroporation of plasmid DNA and thus open new avenues of discovery research in skeletal muscle physiology.

Publication Title

Journal of applied physiology (Bethesda, Md. : 1985)

Volume

133

Issue

1

First Page

41

Last Page

59

Digital Object Identifier (DOI)

10.1152/japplphysiol.00088.2022

PubMed ID

35511722

E-ISSN

1522-1601

Language

eng

Citation Information

Hughes, David C.; Hardee, Justin P.; Waddell, David S.; and Goodman, Craig A., "CORP: Gene delivery into murine skeletal muscle using in vivo electroporation" (2022). UNF Faculty Research and Scholarship. 3209.
https://digitalcommons.unf.edu/unf_faculty_publications/3209