Effects of Aquatic Acidification on Calcium Uptake in White River Shrimp Litopenaeus setiferus Gills
College of Arts and Sciences
Master of Science in Biology (MS)
NACO controlled Corporate Body
University of North Florida. Department of Biology
Dr. Doria Bowers
Dr. John Hatle
Dr. James Gelsleichter
Dr. Cliff Ross
Dr. George Rainbolt
Previous research regarding aquatic acidification has examined the protonation of the carbonate and does not consider calcium to be a limiting factor. This is the first study to suggest that pH may affect the uptake of calcium in crustacean gills. This project describes ion transport mechanisms present in the cell membranes of white river shrimp Litopenaeus setiferus gill epithelium, and the effects of pH on the uptake of calcium by these means. Partially purified membrane vesicles (PPMV) of shrimp gills were prepared through a homogenization process that has been used previously to define ion transport in crab and lobster gill tissues. In the current study, shrimp gill PPMV calcium uptake at 50 µM, and 250 µM was greatest at pH 7.0 (p=0.01, p=0.0001). A valinomycin/K+ induced membrane potential (PD) at pH 7.0 significantly increased (p=0.003) calcium uptake from that observed in the absence of a PD. An induced PD at pH 8.0 significantly increased (p=0.003) calcium uptake from that observed in the absence of a PD, however, was not significantly greater than uptake at pH 7.0 in the presence of a PD (p=0.05). Amiloride (2mM) treatments, and amiloride (2mM) + verapamil (100µM) cocktail treatments showed significant decrease in calcium uptake from the control (p=0.03), however, they were not different from each other. This indicates an electrogenic carrier with two driving forces: calcium concentration, and asymmetric exchange stoichiometry.
Jacobs, Maria-Flora, "Effects of Aquatic Acidification on Calcium Uptake in White River Shrimp Litopenaeus setiferus Gills" (2019). UNF Graduate Theses and Dissertations. 870.
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