Year

2022

Season

Fall

Paper Type

Doctoral Dissertation

College

Brooks College of Health

Degree Name

Doctor of Clinical Nutrition (DCN)

Department

Nutrition & Dietetics

NACO controlled Corporate Body

University of North Florida. Department of Nutrition & Dietetics

First Advisor

Andrea Arikawa, PhD, RDN, LDN

Second Advisor

Alireza Jahan-Mihan, PhD, RDN, LDN

Third Advisor

Judith Ochrietor, PhD

Department Chair

Dr. Andrea Arikawa

College Dean

Dr. Curt Lox

Abstract

This study investigated the impact of regular consumption of fermented vegetables on markers of inflammation and the composition of the gut microbiota in adults at increased risk for cardiovascular disease. Eighty-seven adults ages 35-64 were randomized into a Fermented Vegetable (FV) (100g fermented vegetables daily at least 5x/wk), or a Usual Diet (UD) group. Blood and stool samples were obtained before and after the intervention for measurement of C-Reactive Protein, interleukin-6, angiopoietin-like protein 4, trimethylamine oxide, and lipopolysaccharide binding protein. Next-generation sequencing of the V4 region of the 16S rRNA gene isolated from stool samples was performed on the Illumina MiSeq 500 platform. There were no changes in inflammatory markers or bacterial alpha diversity between groups. LEfSe results indicated that eight bacterial taxa were increased in the FV group compared with two in UD, while 12 decreased in the FV group compared with one in the UD group. Our findings indicate that consuming 100g of fermented vegetables daily for 8 weeks does not change inflammatory biomarkers but does alter the composition of the gut microbiota. Future trials investigating a wider variety of probiotic foods and a longer study time are necessary to determine if a greater effect can be seen.

Available for download on Monday, December 16, 2024

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