Faculty Sponsor
Dr. Judith D. Ochrietor
Faculty Sponsor College
College of Arts and Sciences
Faculty Sponsor Department
Biology
Location
SOARS Virtual Conference
Presentation Website
https://unfsoars.domains.unf.edu/2021/posters/characterization-of-the-expression-of-basigin-and-monocarboxylate-transporters-1-and-4-in-aged-mouse-brains-in-response-to-acute-and-chronic-inflammation/
Keywords
SOARS (Conference) (2021 : University of North Florida) – Archives; SOARS (Conference) (2021 : University of North Florida) – Posters; University of North Florida -- Students -- Research – Posters; University of North Florida. Office of Undergraduate Research; University of North Florida. Graduate School; College students – Research -- Florida – Jacksonville – Posters; University of North Florida – Undergraduates -- Research – Posters; University of North Florida. Department of Biology -- Research – Posters; Honorable Mention Award
Abstract
Project of Merit Winner
Chronic inflammation, or prolonged activation of the immune system, is a major hallmark of many age-related diseases, including heart disease, COVID-19, and neuroinflammation. Typically, the central nervous system (CNS) is protected by a barrier that forms through the coordinated efforts of blood vessel endothelial cells, astrocytes, and pericytes. However, as that barrier breaks down because of chronic inflammation, neuroinflammation can occur. Blood vessel endothelial cells express membrane association proteins, including the cell adhesion molecule Basigin and the transporter protein Monocarboxylate transporter 1 (MCT1). A related MCT protein named MCT4 often increases in expression during chronic inflammation. Basigin associates with both MCT1 and MCT4 in the neural retina. Therefore, the expression of these three proteins (Basigin, MCT1, and MCT4) was investigated in response to chronic inflammation in aged mouse brains. It was hypothesized that the expression of Basigin and MCT1 would decrease and that of MCT4 would increase in response to chronic inflammation. The brains of 6-month-old mice were harvested and exposed to lipopolysaccharide (LPS; 10 g/mL), a known activator of inflammation, or saline control for 3, 6, 12, or 24 hours. The brain proteins were isolated and subjected to ELISA analyses designed to measure each target protein. It was determined that no significant difference in Basigin, MCT1, or MCT4 expression was observed for any treatment time. The hypothesis was not supported, and the data suggest that Basigin, MCT1, and MCT4 may not contribute to the development of neuroinflammatory diseases.
Rights Statement
http://rightsstatements.org/vocab/InC/1.0/
Included in
Characterization of the expression of Basigin and Monocarboxylate Transporters 1 and 4 in aged mouse brains in response to acute and chronic inflammation
SOARS Virtual Conference
Project of Merit Winner
Chronic inflammation, or prolonged activation of the immune system, is a major hallmark of many age-related diseases, including heart disease, COVID-19, and neuroinflammation. Typically, the central nervous system (CNS) is protected by a barrier that forms through the coordinated efforts of blood vessel endothelial cells, astrocytes, and pericytes. However, as that barrier breaks down because of chronic inflammation, neuroinflammation can occur. Blood vessel endothelial cells express membrane association proteins, including the cell adhesion molecule Basigin and the transporter protein Monocarboxylate transporter 1 (MCT1). A related MCT protein named MCT4 often increases in expression during chronic inflammation. Basigin associates with both MCT1 and MCT4 in the neural retina. Therefore, the expression of these three proteins (Basigin, MCT1, and MCT4) was investigated in response to chronic inflammation in aged mouse brains. It was hypothesized that the expression of Basigin and MCT1 would decrease and that of MCT4 would increase in response to chronic inflammation. The brains of 6-month-old mice were harvested and exposed to lipopolysaccharide (LPS; 10 g/mL), a known activator of inflammation, or saline control for 3, 6, 12, or 24 hours. The brain proteins were isolated and subjected to ELISA analyses designed to measure each target protein. It was determined that no significant difference in Basigin, MCT1, or MCT4 expression was observed for any treatment time. The hypothesis was not supported, and the data suggest that Basigin, MCT1, and MCT4 may not contribute to the development of neuroinflammatory diseases.
https://digitalcommons.unf.edu/soars/2021/spring_2021/41