Development of a clickable activity-based protein profiling (ABPP) probe for agmatine deiminases
Agmatine deiminases (AgDs) catalyze the hydrolytic conversion of agmatine (decarboxylated arginine) to N-carbamoylputrescine with concomitant release of ammonia. These enzymes, which are encoded by some pathogenic bacterial species, confer a competitive survival advantage by virtue of energy production and acid tolerance through agmatine catabolism. Herein we report the development of a clickable activity-based protein profiling (ABPP) probe that targets the AgD encoded by Streptococcus mutans with high selectivity and sensitivity.
Bioorganic and Medicinal Chemistry
Digital Object Identifier (DOI)
Marchenko, Thomson, A., Ellis, T. N., Knuckley, B., & Causey, C. P. (2015). Development of a clickable activity-based protein profiling (ABPP) probe for agmatine deiminases. Bioorganic & Medicinal Chemistry, 23(9), 2159–2167. https://doi.org/10.1016/j.bmc.2015.03.013