Design, synthesis, and in vitro evaluation of an activity-based protein profiling (ABPP) probe targeting agmatine deiminases

Document Type

Article

Publication Date

9-1-2014

Abstract

Agmatine deiminases (AgDs) belong to a family of enzymes known as guanidinium group modifying enzymes (GMEs). Many pathogenic bacteria encode an AgD that participates in the catabolism of agmatine (decarboxylated arginine). This catabolism may confer a competitive survival advantage, by virtue of energy production and increased acid tolerance, making this sub-family of enzymes a potential therapeutic target that warrants further study. Herein we report the development of an activity-based protein profiling (ABPP) probe that selectively targets the AgD from Streptococcus mutans. Due to the selectivity and covalent nature of the modification, this probe could prove to be a valuable tool for the study of other AgD family members. © 2014 Elsevier Ltd. All rights reserved.

Publication Title

Bioorganic and Medicinal Chemistry

Volume

22

Issue

17

First Page

4602

Last Page

4608

Digital Object Identifier (DOI)

10.1016/j.bmc.2014.07.028

PubMed ID

25127464

ISSN

09680896

E-ISSN

14643391

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