Efficient bioconversion of lignocellulosic waste by a novel computationally screened hyperthermostable enzyme from a specialized microbiota

Authors

Shohreh Ariaeenejad, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO)
Kaveh Kavousi, Institute of Biochemistry and Biophysics (IBB), University of Tehran
Behrouz Zolfaghari, Indian Institute of Technology (IIT), Haliç University Eyüpsultan, Istanbul.
Swapnoneel Roy, University of North FloridaFollow
Takeshi Koshiba, Waseda University, Japan.
Ghasem Hosseini Salekdeh, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research Education and Extension Organization (AREEO), Karaj, Iran; Macquarie University, Sydney, Australia.

Document Type

Article

Publication Date

2-7-2023

Abstract

A large amount of lignocellulosic waste is generated every day in the world, and their accumulation in the agroecosystems, integration in soil compositions, or incineration for energy production has severe environmental pollution effects. Using enzymes as biocatalysts for the biodegradation of lignocellulosic materials, especially in harsh processing conditions, is a practical step towards green energy and environmental biosafety. Hence, the current study focuses on enzyme computationally screened from camel rumen metagenomics data as specialized microbiota that have the capacity to degrade lignocellulosic-rich and recalcitrant materials. The novel hyperthermostable xylanase named PersiXyn10 with the performance at extreme conditions was proper activity within a broad temperature (30-100 ℃) and pH range (4.0-11.0) but showed the maximum xylanolytic activity in severe alkaline and temperature conditions, pH 8.0 and temperature 90 ℃. Also, the enzyme had highly resistant to metals, surfactants, and organic solvents in optimal conditions. The introduced xylanase had unique properties in terms of thermal stability by maintaining over 82% of its activity after 15 days of incubation at 90 ℃. Considering the crucial role of hyperthermostable xylanases in the paper industry, the PersiXyn10 was subjected to biodegradation of paper pulp. The proper performance of hyperthermostable PersiXyn10 on the paper pulp was confirmed by structural analysis (SEM and FTIR) and produced 31.64 g/L of reducing sugar after 144 h hydrolysis. These results proved the applicability of the hyperthermostable xylanase in biobleaching and saccharification of lignocellulosic biomass for declining the environmental hazards.

Publication Title

Ecotoxicology and environmental safety

Volume

252

First Page

114587

Digital Object Identifier (DOI)

10.1016/j.ecoenv.2023.114587

PubMed ID

36758508

E-ISSN

1090-2414

Language

eng

Citation Information

Ariaeenejad, Shohreh; Kavousi, Kaveh; Zolfaghari, Behrouz; Roy, Swapnoneel; Koshiba, Takeshi; and Hosseini Salekdeh, Ghasem, "Efficient bioconversion of lignocellulosic waste by a novel computationally screened hyperthermostable enzyme from a specialized microbiota" (2023). UNF Faculty Research and Scholarship. 3213.
https://digitalcommons.unf.edu/unf_faculty_publications/3213