Biolayer Interferometry: A Novel Method to Elucidate Protein-Protein and Protein-DNA Interactions in the Mitochondrial DNA Replisome
Document Type
Article
Publication Date
1-1-2016
Subject Area
Biosensing Techniques (methods); DNA Polymerase gamma; DNA Replication (genetics); DNA, Mitochondrial (genetics); DNA, Single-Stranded (genetics, metabolism); DNA-Binding Proteins (metabolism); DNA-Directed DNA Polymerase (genetics); Humans; Interferometry (methods); Mitochondria (genetics); Mitochondrial Diseases (genetics); Multienzyme Complexes (genetics)
Abstract
A lack of effective treatment for mitochondrial diseases prompts scientists to investigate the molecular processes that underlie their development. The major cause of mitochondrial diseases is dysfunction of the sole mitochondrial DNA polymerase, DNA polymerase γ (Pol γ). The development of treatment strategies will require a detailed characterization of the molecular properties of Pol γ. A novel technique, biolayer interferometry, allows one to monitor molecular interactions in real time, thus providing an insight into the kinetics of the process. Here, we present an application of the biolayer interferometry technique to characterize the fundamental reactions that Pol γ undergoes during the initiation phase of mitochondrial DNA replication: holoenzyme formation and binding to the primer-template.
Publication Title
Methods in molecular biology (Clifton, N.J.)
Volume
1351
First Page
223
Last Page
31
Digital Object Identifier (DOI)
10.1007/978-1-4939-3040-1_17
PubMed ID
26530686
E-ISSN
1940-6029
Language
eng
Citation Information
Ciesielski, Grzegorz L.; Hytönen, Vesa P.; and Kaguni, Laurie S., "Biolayer Interferometry: A Novel Method to Elucidate Protein-Protein and Protein-DNA Interactions in the Mitochondrial DNA Replisome" (2016). UNF Faculty Research and Scholarship. 3289.
https://digitalcommons.unf.edu/unf_faculty_publications/3289