Year

2024

Season

Summer

Paper Type

Master's Thesis

College

College of Arts and Sciences

Degree Name

Master of Science in Biology (MS)

Department

Biology

NACO controlled Corporate Body

University of North Florida. Department of Biology

Committee Chairperson

Dr. Dale A. Casamatta

Second Advisor

Dr. Eric G. Johnson

Rights Statement

http://rightsstatements.org/vocab/InC/1.0/

Third Advisor

Dr. Kelly J. Smith

Department Chair

Dr. Cliff Ross

College Dean

Dr. Kaveri Subrahmanyam

Abstract

Abstract

Freshwater harmful algal blooms (HABs) are a global water quality issue. Algae, especially cyanobacteria, are integrally associated with complex microbial assemblages, which cycle nutrients, provide microenvironments, breakdown potentially harmful molecules, etc. However, these same microbial consortia are competitors or even inhibitors to algae in these systems. One potential biological control is Rhodospirillum rubrum, a Gram- proteobacterium. To evaluate its capacity for HAB mitigation, experimental mesocosms were inoculated with a native assortment of algae and R. rubrum and grown for 2-months. Relative Fluorescence Units (RFU), Ash Free Dry Mass, and eukaryotic (23S rDNA) metabarcoding were conducted to estimate algal abundance and community composition. The RFU was influenced by treatment (Kruskal-Wallis, pR. rubrum addition (p>0.05), and R. rubrum lowered algal abundance directly after application. However, more studies on R. rubrum are needed before application as a biological control, but the employment of R. rubrum may be a potential control in some HAB scenarios.

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