Green tea catechin EGCG could prevent obesity-related precocious puberty through NKB/NK3R signaling pathway
Document Type
Article
Publication Date
10-1-2022
Subject Area
Animals; Camellia sinensis (chemistry); Catechin (administration & dosage, analogs & derivatives, pharmacology); Female; Humans; Neurokinin B (genetics, metabolism); Obesity (complications); Puberty, Precocious (etiology, prevention & control); Rats; Retrospective Studies; Signal Transduction
Abstract
This study aimed to explore the potential regulatory pathways of (-)-epigallocatechin-3-gallate (EGCG) in preventing obesity-related precocious puberty. A retrospective analysis on the impact of EGCG on puberty onset in obese girls was conducted on plasma samples collected from a human randomized controlled trial. In the trial, participants consumed EGCG capsules for 12 weeks. In the animal experiment, rats were divided into four groups: normal diet control (NC) group, high-fat diet (HFD) group, NC+EGCG group, and HFD+EGCG group. Blood samples were collected on postnatal days 27, 33, and 36 to detect sexual development indicators. The hypothalamic expressions of kisspeptin/Kiss1R and neurokinin B (NKB)/NK3R signaling were measured by RT-qPCR and Western blot assay. The ovary NKB protein expression was assessed by immunohistochemical assays. Serum NKB level in the EGCG group was lower than the placebo group by 0.599 ng/mL [β=-0.599, 95% CI: (-1.005, -0.193)], at the end of intervention and after adjusting for confounders (clinical study). In the animal experiment, EGCG intervention could significantly delay the vaginal opening (VO) time of rats fed with HFD. On day 33, EGCG intervention could significantly reduce serum NKB, luteinizing hormone (LH) levels, ovarian NKB protein expression, and endometrial thickness of HFD-fed rats, while EGCG intervention could remarkably increase mRNA and protein expression of NKB/NK3R. EGCG could prevent obesity-related precocious puberty through NKB/NK3R signaling pathway, which may provide a novel insight into the role of EGCG in preventing precocious puberty in obese girls.
Publication Title
The Journal of nutritional biochemistry
Volume
108
First Page
109085
Digital Object Identifier (DOI)
10.1016/j.jnutbio.2022.109085
PubMed ID
35691596
E-ISSN
1873-4847
Language
eng
Citation Information
Gu, Qiuyun; Wang, Xiaodi; Xie, Luyao; Yao, Xinyuan; Qian, Linxi; Yu, Zhiping; and Shen, Xiuhua, "Green tea catechin EGCG could prevent obesity-related precocious puberty through NKB/NK3R signaling pathway" (2022). UNF Faculty Research and Scholarship. 3175.
https://digitalcommons.unf.edu/unf_faculty_publications/3175